Delineation and modelling of a nucleolar retention signal in the coronavirus nucleocapsid protein.
Identifieur interne : 003F29 ( Main/Exploration ); précédent : 003F28; suivant : 003F30Delineation and modelling of a nucleolar retention signal in the coronavirus nucleocapsid protein.
Auteurs : Mark L. Reed [Royaume-Uni] ; Brian K. Dove ; Richard M. Jackson ; Rebecca Collins ; Gavin Brooks ; Julian A. HiscoxSource :
- Traffic (Copenhagen, Denmark) [ 1398-9219 ] ; 2006.
Descripteurs français
- KwdFr :
- Alignement de séquences, Animaux, Biologie informatique, Cellules Vero, Données de séquences moléculaires, Leucine (génétique), Leucine (métabolisme), Modèles moléculaires, Nucléole (métabolisme), Protéines nucléocapside (), Protéines nucléocapside (génétique), Protéines nucléocapside (métabolisme), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Signaux de localisation nucléaire (), Signaux de localisation nucléaire (génétique), Signaux de localisation nucléaire (physiologie), Structure tertiaire des protéines, Séquence conservée, Transport nucléaire actif, Virus de la bronchite infectieuse (), Virus de la bronchite infectieuse (génétique), Virus de la bronchite infectieuse (métabolisme).
- MESH :
- génétique : Leucine, Protéines nucléocapside, Protéines recombinantes, Signaux de localisation nucléaire, Virus de la bronchite infectieuse.
- métabolisme : Leucine, Nucléole, Protéines nucléocapside, Protéines recombinantes, Virus de la bronchite infectieuse.
- physiologie : Signaux de localisation nucléaire.
- Alignement de séquences, Animaux, Biologie informatique, Cellules Vero, Données de séquences moléculaires, Modèles moléculaires, Protéines nucléocapside, Signaux de localisation nucléaire, Structure tertiaire des protéines, Séquence conservée, Transport nucléaire actif, Virus de la bronchite infectieuse.
English descriptors
- KwdEn :
- Active Transport, Cell Nucleus, Animals, Cell Nucleolus (metabolism), Chlorocebus aethiops, Computational Biology, Conserved Sequence, Infectious bronchitis virus (chemistry), Infectious bronchitis virus (genetics), Infectious bronchitis virus (metabolism), Leucine (genetics), Leucine (metabolism), Models, Molecular, Molecular Sequence Data, Nuclear Localization Signals (chemistry), Nuclear Localization Signals (genetics), Nuclear Localization Signals (physiology), Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (metabolism), Protein Structure, Tertiary, Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Sequence Alignment, Vero Cells.
- MESH :
- chemical , chemistry : Nuclear Localization Signals, Nucleocapsid Proteins.
- chemical , genetics : Leucine, Nuclear Localization Signals, Nucleocapsid Proteins, Recombinant Proteins.
- chemistry : Infectious bronchitis virus.
- genetics : Infectious bronchitis virus.
- metabolism : Cell Nucleolus, Infectious bronchitis virus, Leucine, Nucleocapsid Proteins, Recombinant Proteins.
- chemical , physiology : Nuclear Localization Signals.
- Active Transport, Cell Nucleus, Animals, Chlorocebus aethiops, Computational Biology, Conserved Sequence, Models, Molecular, Molecular Sequence Data, Protein Structure, Tertiary, Sequence Alignment, Vero Cells.
Abstract
Unlike nuclear localization signals, there is no obvious consensus sequence for the targeting of proteins to the nucleolus. The nucleolus is a dynamic subnuclear structure which is crucial to the normal operation of the eukaryotic cell. Studying nucleolar trafficking signals is problematic as many nucleolar retention signals (NoRSs) are part of classical nuclear localization signals (NLSs). In addition, there is no known consensus signal with which to inform a study. The avian infectious bronchitis virus (IBV), coronavirus nucleocapsid (N) protein, localizes to the cytoplasm and the nucleolus. Mutagenesis was used to delineate a novel eight amino acid motif that was necessary and sufficient for nucleolar retention of N protein and colocalize with nucleolin and fibrillarin. Additionally, a classical nuclear export signal (NES) functioned to direct N protein to the cytoplasm. Comparison of the coronavirus NoRSs with known cellular and other viral NoRSs revealed that these motifs have conserved arginine residues. Molecular modelling, using the solution structure of severe acute respiratory (SARS) coronavirus N-protein, revealed that this motif is available for interaction with cellular factors which may mediate nucleolar localization. We hypothesise that the N-protein uses these signals to traffic to and from the nucleolus and the cytoplasm.
DOI: 10.1111/j.1600-0854.2006.00424.x
PubMed: 16734668
Affiliations:
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Le document en format XML
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The nucleolus is a dynamic subnuclear structure which is crucial to the normal operation of the eukaryotic cell. Studying nucleolar trafficking signals is problematic as many nucleolar retention signals (NoRSs) are part of classical nuclear localization signals (NLSs). In addition, there is no known consensus signal with which to inform a study. The avian infectious bronchitis virus (IBV), coronavirus nucleocapsid (N) protein, localizes to the cytoplasm and the nucleolus. Mutagenesis was used to delineate a novel eight amino acid motif that was necessary and sufficient for nucleolar retention of N protein and colocalize with nucleolin and fibrillarin. Additionally, a classical nuclear export signal (NES) functioned to direct N protein to the cytoplasm. Comparison of the coronavirus NoRSs with known cellular and other viral NoRSs revealed that these motifs have conserved arginine residues. Molecular modelling, using the solution structure of severe acute respiratory (SARS) coronavirus N-protein, revealed that this motif is available for interaction with cellular factors which may mediate nucleolar localization. We hypothesise that the N-protein uses these signals to traffic to and from the nucleolus and the cytoplasm.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Yorkshire-et-Humber</li></region><settlement><li>Leeds</li></settlement><orgName><li>Université de Leeds</li></orgName></list><tree><noCountry><name sortKey="Brooks, Gavin" sort="Brooks, Gavin" uniqKey="Brooks G" first="Gavin" last="Brooks">Gavin Brooks</name><name sortKey="Collins, Rebecca" sort="Collins, Rebecca" uniqKey="Collins R" first="Rebecca" last="Collins">Rebecca Collins</name><name sortKey="Dove, Brian K" sort="Dove, Brian K" uniqKey="Dove B" first="Brian K" last="Dove">Brian K. Dove</name><name sortKey="Hiscox, Julian A" sort="Hiscox, Julian A" uniqKey="Hiscox J" first="Julian A" last="Hiscox">Julian A. Hiscox</name><name sortKey="Jackson, Richard M" sort="Jackson, Richard M" uniqKey="Jackson R" first="Richard M" last="Jackson">Richard M. Jackson</name></noCountry><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Reed, Mark L" sort="Reed, Mark L" uniqKey="Reed M" first="Mark L" last="Reed">Mark L. Reed</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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